產(chǎn)品概述

白介素 2（IL-2）是通過結(jié)合多分子細(xì)胞受體復(fù)合物發(fā)揮生物活力的。多年來，這個受體復(fù)合物被認(rèn)為是由IL-2Rα與IL-2β兩個糖蛋白鏈組成的，兩個亞基相互結(jié)合在一起形成一個高親和力受體來傳遞 IL-2信號的。IL-2Rα（也稱為 Tac 抗原或 CD25），是一個分子量為55kD跨膜糖蛋白，有351個氨基酸組成。IL-2β在1989 年被克隆出來，它是個由575個氨基酸組成的跨膜糖蛋白，分子量為75kD，其中有286個氨基酸位于胞漿內(nèi)，更顯著地參與了細(xì)胞信號傳導(dǎo)作用。常認(rèn)為它是IL-2一種弱抑制劑。在任何情況下，體液中增高的可溶性IL-2Rα常伴隨這T細(xì)胞與B細(xì)胞的增加與免疫系統(tǒng)的激活。

產(chǎn)品原理

本實驗采用雙抗體夾心ELISA法?？谷薎L-2 sRα 單抗包被于酶標(biāo)板上，標(biāo)本和標(biāo)準(zhǔn)品中的IL-2 sRα 會與單抗結(jié)合形成免疫復(fù)合物，游離的成分被洗去；加入生物素化的抗人IL-2 sRα 抗體，生物素化抗人IL-2 sRα 抗體與酶標(biāo)板上結(jié)合的標(biāo)準(zhǔn)品或樣本中的IL-2 sRα 結(jié)合而形成免疫復(fù)合物，游離的成分被洗去；加入辣根過氧化物酶標(biāo)記的親合素，親合素與生物素特異性結(jié)合，游離的成分被洗去。加入顯色底物（顯色劑），若反應(yīng)孔中有IL-2 sRα，辣根過氧化物酶會使無色的顯色劑變藍(lán)，加終止液變黃。在450nm處測OD值，IL-2 sRα 濃度與OD₄₅₀值之間呈正相關(guān)，可通過繪制標(biāo)準(zhǔn)曲線求出標(biāo)本中IL-2 sRα 濃度。

產(chǎn)品性能

1. 靈敏度：最小可測人IL-2 sRα達(dá)15pg/ml 。

2. 重復(fù)性：板內(nèi)、板間變異系數(shù)均<10%。

產(chǎn)品組分及儲存條件

試驗所需自備儀器和試劑:

1. 酶標(biāo)儀(450nm檢測波長濾光片，570nm或630nm校正波長濾光片)。

2. 高精度加液器及一次性吸頭：0.5-10μl，2-20μl，20-200μl，200-1000μl。

3. 37℃恒溫箱、蒸餾水或去離子水、坐標(biāo)紙。

• HUMAN Insulin ELISA KIT（EH046-96）
• HUMAN IL-5 ELISA KIT（EH044-96）
• HUMAN VCAM-1 ELISA KIT（EH042-96）

• ELISA kit 產(chǎn)品手冊2019點擊下載

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He W et al. Vitamin B5 ReducesBacterialGrowth via Regulating Innate Immunity and Adaptive Immunity in MiceInfectedwith Mycobacterium tuberculosis. Front Immunol, 2018. Read more (Pubmed :29535733)

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He MC et al. Paricalcitolalleviateslipopolysaccharide-induced depressive-like behavior by suppressinghypothalamicmicroglia activation and neuroinflammation.  Biochem Pharmacol,2019. Read More (Pubmed :30703351)

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