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ResiQuant? Ready-to-use E.coli DNA Residue Detection Kit Production Introduce（CRH00-1101）

ResiQuant? Ready-to-use E.coli DNA Residue Detection Kit Production Introduce（CRH00-1101）

ResiQuant? Ready-to-use E.coli DNA Residue Detection Kit Production Introduce


Catalog No.
Size
CRH00-1101
 50T
CRH00-1102
 100T

Overview performance Related application image Data and files literature Q&A COA

This kit uses the Taqman probe quantitative PCR method to quickly detect residual Escherichia coli (E.coli) host DNA in intermediates, semi-finished products and finished products of various biological products and pharmaceuticals. The kit introduces an uracil-N-glycosylase (UNG) system, which can effectively remove residual contamination of PCR products and great reduce false positives caused by contamination of amplified products. The reagent components contain internal standard control (IC), we can monitor whether the reaction process is proceeding normally and eliminate sample interference; ROX is suitable for ABI fluorescence quantitative PCR instruments or other similar equipment, and plays a role in optical path correction.
This product is suitable for biology drug solutions, finished products, semi-finished products, intermediate products or process monitoring samples produced with Escherichia coli stains (such as E.coli-K12，E.coli-DH5α，E.coli-MC1061，E.coli-HB101，E.coli-AM-7，E.coli-XL1-Blue，E.coli-YK537, etc.) as host. To obtain a calibration curve for quantitative sample DNA, the kit contains DNA calibrators with 5 concentration gradients (300 pg/μL to 30 fg/μL) for preparation of calibration curve.
For the extraction of residual DNA from E.coli host bacteria, it is recommended to use our company’s universal residual host DNA sample pretreatment kit. Before first use, it is recommended to complete a product suitability study: confirm whether there are interferences or inhibitors in the sample or sample matrix, and select appropriate sample detection dilution conditions.

Product Features
1.Quantitative Range: 30 fg/μL  to 300 pg/μL；
2.Accuracy: DNA calibrators have a complete traceability chain, traceable to national standards；
3.Precision: high intra-batch repeatability and small inter-batch variation within the quantitative limit;
4.Exclusive: Specific detection of residual DNA in E.coli host cells without interference from 293,Vero,CHO and other foreign cells;
5.Simplicity: Ready-to-use calibrators eliminate the need for cumbersome dilution operations, simplifying the experimental process; avoiding artificial differences in standard curve dilution operations, and achieving good consistency of results.
6.Anti-interference: UNG anti-pollution system effectively removes PCR product contamination；
Internal Control（IC）to improve detection control of sample interference and reaction preparation；

Product Advantages
Strong Anti-interference
UNG Anti-pollution System and Digestion Procedure：Improve the kit’s tolerance to aerosol contamination of PCR products；
Internal Control (IC): Improve detection control of sample interference and reaction preparation;
Ready-to-use calibrator
No need for cumbersome dilution operations, simplifying the experimental process; avoid artificial differences in the standard dilution operation, and the results are consistent;
Complete kit traceability system
The DNA calibrator has a complete traceability chain, trace to the national standard material, the kit traceability system is complete;
Supply chain security
Large batch output, short delivery time, stable supply;