產(chǎn)品概述
白介素-1(IL-1)是IL-1α和IL-1β 兩種蛋白的統(tǒng)稱,是由不同基因編碼的,但它們識別細胞表面相同的受體。IL-1α和IL-1β結(jié)構(gòu)相似,在氨基酸水平上有25%的同源性;兩種都是由分子量31kDa 前體裂解成17.5kDa的蛋白質(zhì)。IL-1α和IL-1β都不包含典型的疏水信號肽,但是有證據(jù)證明一些非典型路徑可以分泌這些因子。大量的IL-1α以前體形式保留在胞內(nèi)。一部分未修飾的IL-1α運輸?shù)郊毎砻娴耘c細胞相連。未修飾的膜結(jié)合IL-1α體現(xiàn)生物活性,鄰近的細胞旁分泌IL-1。 IL-1α和IL-1β與特定的受體結(jié)合后發(fā)揮其作用。
產(chǎn)品原理
本實驗采用雙抗體夾心ELISA法??谷薎L-1α單抗包被于酶標板上,標本和標準品中的IL-1α會與單抗結(jié)合形成免疫復合物,游離的成分被洗去;加入生物素化的抗人IL-1α抗體,生物素化抗人IL-1α抗體與酶標板上結(jié)合的標準品或樣本中的IL-1α結(jié)合而形成免疫復合物,游離的成分被洗去;加入辣根過氧化物酶標記的親合素,親合素與生物素特異性結(jié)合,游離的成分被洗去。加入顯色底物(顯色劑),若反應孔中有IL-1α,辣根過氧化物酶會使無色的顯色劑變藍,加終止液變黃。在450nm處測OD值, IL-1α 濃度與OD450值之間呈正相關(guān),可通過繪制標準曲線求出標本中IL-1α濃度。
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